Ag85B from M. tuberculosis H37Rv |
|
Gene Name |
Ag85B from M. tuberculosis H37Rv |
|
Sequence Strain (Species/Organism) |
Mycobacterium tuberculosis H37Rv |
|
VO ID |
VO_0012364
|
|
NCBI Gene ID |
885785
|
|
NCBI Protein GI |
15609023
|
|
Locus Tag |
Rv1886c |
|
Genbank Accession |
AL123456 |
|
Protein Accession |
NP_216402 |
|
Other Database IDs |
EMBL: AE000516
EMBL: AF198032
EMBL: BX842578
EMBL: U38939
EMBL: X62398
GenomeReviews: AE000516_GR
GenomeReviews: AL123456_GR
InterPro: IPR000801
KEGG: mtc:MT1934
KEGG: mtu:Rv1886c
PDB: 1F0N
PDB: 1F0P
Pfam: PF00756
PIR: C70516
TIGR: MT1934
TubercuList: Rv1886c
UniProt: P31952 |
|
Taxonomy ID |
83332
|
|
Gene Starting Position |
2134889 |
|
Gene Ending Position |
2135866 |
|
Gene Strand (Orientation) |
- |
|
Protein Name |
diacylglycerol acyltransferase/mycolyltransferase Ag85B |
|
Protein pI |
5.72 |
|
Protein Weight |
33091.46 |
|
Protein Length |
325 |
|
Protein Note |
Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B |
|
Protein Annotation |
FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P31952)
SUBCELLULAR LOCATION: Secreted protein (UniProt: P31952)
SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P31952) |
|
DNA Sequence |
>NC_000962.3:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome
GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA
GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG
AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT
TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT
AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG
TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC
CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC
GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG
AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC
TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT
GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC
CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG
GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC
TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA
|
|
Protein Sequence |
>NP_216402.1 diacylglycerol acyltransferase/mycolyltransferase Ag85B [Mycobacterium tuberculosis H37Rv]
MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG
NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD
AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF
QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG
|
|
Molecule Role |
Protective antigen |
|
Molecule Role Annotation |
In mice, a primary vaccination with Ag85B-encoding plasmid DNA (DNA-85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B-specific CD4+ T cells producing IFN-gamma and controlling intramacrophagic MTB growth (Palma et al., 2007). |
| Related Vaccines(s) |
AER in PLGA nanoparticles + CpG + MPLA
,
Ag85B and ESAT-6 fusion protein
,
Ag85B:HspX:hFcγ1
,
AR2+DMC
,
Arabinomannan-tetanus toxoid conjugate
,
ARM
,
ERA005f
,
H1 / CAF01
,
H4-IC31
,
H56:IC31
,
LT-BCG- Ag85B /Rv3425
,
M. tuberculosis DNA Vaccine encoding Ag85B Protein
,
M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83
,
MVA/IL-15/5Mtb vaccine
,
rBCG-Ag85A/ Ag85B
,
Spore-FP1
|
| References |
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