• Vaccine Name: rBCG-Ag85A/ Ag85B
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0004757
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• FbpA (Ag85A) gene engineering:
  • Type: Recombinant vector construction
  • Description: rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012).
  • Detailed Gene Information: Click Here.
• Ag85B from M. tuberculosis H37Rv gene engineering:
  • Type: Recombinant protein preparation
  • Description: rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012).
  • Detailed Gene Information: Click Here.
• Vector: BCG vaccine vector
  • Vector name: BCG vaccine vector
  • VO vector ID: VO_0001023
• Preparation: Three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis (Wang et al., 2012).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Mice in each group were immunized s.c. once at the base of the tail with 1 × 106 CFU of either rBCG::85A, rBCG::85B, or rBCG::AB in a final volume of 100 μL of PBS (Wang et al., 2012).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: mice were challenged with virulent M. tuberculosis strain 6 weeks after immunization (Wang et al., 2012).
• Efficacy: rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses (Wang et al., 2012).
• Information about this animal model: Mouse Model for TB research