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Vaccine Detail
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Ag85B:HspX:hFc?1 |
| Vaccine Information |
- Vaccine Name: Ag85B:HspX:hFc?1
- Target Pathogen: Mycobacterium tuberculosis
- Target Disease: Tuberculosis
- Type: Subunit vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: Ag85B:HspX:hFc?1
- Ag85B from M. tuberculosis H37Rv
gene engineering:
- Type: Fusion Protein
- Description: Use in fusion protein preparation(Karbalaei et al., 2024).
- Detailed Gene Information: Click Here.
- hspX
gene engineering:
- Type: Fusion Protein
- Description: Use in fusion protein preparation(Karbalaei et al., 2024).
- Detailed Gene Information: Click Here.
- Preparation: The vaccine consists of a recombinant protein expressed in Pichia pastoris, purified by Protein A affinity chromatography, and formulated as a protein subunit vaccine(Karbalaei et al., 2024).
- Immunization Route: subcutaneous injection
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| Host Response |
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Mouse Response
- Host Strain: C57BL/6
- Vaccination Protocol: Subcutaneous immunization of mice with Ag85B:HspX:hFc?1 formulated with DDA/TDB, administered four times at two-week intervals (Karbalaei et al., 2024).
- Immune Response: Vaccination induced strong Th1 responses characterized by increased IFN-? and IL-12 expression, with moderate TGF-? production and no significant IL-4 induction (Karbalaei et al., 2024).
- Information about this animal model: Mouse Model for TB research
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| References |
Karbalaei et al., 2024: Karbalaei M, Mosavat A, Soleimanpour S, Farsiani H, Ghazvini K, Amini AA, Sankian M, Rezaee SA. Production and Evaluation of Ag85B:HspX:hFcγ1 Immunogenicity as an Fc Fusion Recombinant Multi-Stage Vaccine Candidate Against Mycobacterium tuberculosis. Current microbiology. 2024; 81(5); 127. [PubMed: 38575759].
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