• Vaccine Name: H1 / CAF01
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Mouse
• Antigen: H1 fusion protein composed of Ag85B and ESAT-6 from Mycobacterium tuberculosis (Klessing et al., 2020).
• Ag85B from M. tuberculosis H37Rv gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Klessing et al., 2020).
  • Detailed Gene Information: Click Here.
• EsxA (ESAT-6) gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Klessing et al., 2020).
  • Detailed Gene Information: Click Here.
• Adjuvant: CAF01 vaccine adjuvant
  • Adjuvant name: CAF01 vaccine adjuvant
  • VO adjuvant ID: VO_0005483
  • Description: CAF01 was used as the adjuvant in this vaccine to enhance immune responses to the H1 fusion protein antigen (Klessing et al., 2020).
• Preparation: The H1 tuberculosis subunit vaccine was prepared by formulating a recombinant fusion protein consisting of Ag85B and ESAT-6 with the liposomal adjuvant CAF01. Prior to immunization, the H1 antigen was allowed to adsorb to the CAF01 adjuvant for 60 minutes on ice before subcutaneous injection (Klessing et al., 2020).
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: C57BL/6 mice
• Vaccination Protocol: Mice were immunized twice at a three- to four-week interval with the H1 fusion protein formulated in CAF01, followed by additional booster immunizations depending on the experimental design (Klessing et al., 2020).
• Description: The H1/CAF01 vaccine is a subunit fusion protein vaccine composed of a recombinant Ag85B–ESAT-6 fusion protein formulated with the cationic liposomal adjuvant CAF01. In mice, this vaccine induces strong, long-lasting Th1- and Th17-biased immune responses and supports durable antibody and plasma cell formation (Klessing et al., 2020).
• Information about this animal model: Mouse Model for TB research