• Vaccine Name: P. yoelii MSP1 and MSP4/5 Proteins Subunit Vaccine
• Target Pathogen: Plasmodium spp.
• Target Disease: Malaria
• Vaccine Ontology ID: VO_0011439
• Type: Subunit vaccine
• Status: Research
• Host Species as Laboratory Animal Model: mouse
• MSP4/5 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Immunization Route: Orally

Mouse Response

• Vaccination Protocol: One group of mice was treated by gavage with 25 μg of EcMSP4/5, and the other group was treated by gavage with 25 μg of EcMSP4/5 plus an amount of GST-PyMSP119 equivalent to 25 μg of PyMSP119 (Wang et al., 2004).
• Challenge Protocol: In order to examine the protective efficacy of the induced antibodies, the immunized mice were challenged at 2 weeks after the sixth immunization with a lethal dose of 10^5 P. yoelii YM parasites (Wang et al., 2004).
• Efficacy: Oral immunization of mice with Escherichia coli-expressed Plasmodium yoelii merozoite surface protein 4/5 or the C-terminal 19-kDa fragment of merozoite surface protein 1 induced systemic antibody responses and protected mice against lethal malaria infection. All of the eight immunized mice survived the challenge, with peak parasitemia levels between 0.2 and 55.2% (Wang et al., 2004).