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Vaccine Detail

Human papillomavirus DNA vaccine pNGVL4a-E6/opt
Vaccine Information
  • Vaccine Name: Human papillomavirus DNA vaccine pNGVL4a-E6/opt
  • Target Pathogen: Human Papillomavirus
  • Target Disease: HPV infection
  • Vaccine Ontology ID: VO_0011480
  • Type: DNA vaccine
  • Status: Research
  • Antigen: Human papillomavirus E6
  • E6 Type 16 gene engineering:
    • Type: DNA vaccine construction
    • Description: To generate pNGVL4a-E6, E6 was isolated from pcDNA3-E6 and cloned into pNGVL4a vector. To generate pNGVL4a-E6/opt, codonoptimized E6 synthesized by GenScript Corporation (Piscataway, NJ) was cloned into EcoRI/BamHI of pNGVL4a vector. The DNA and amino acid sequences of the wild-type E6 gene as well as the codon-optimized E6 were fully sequenced (Lin et al., 2006).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid:
    • DNA vaccine plasmid name:
    • DNA vaccine plasmid VO ID: VO_0005014
  • Immunization Route: Intradermal injection (i.d.)
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: Gold particles coated with pNGVL4a, pNGVL4a-E6 or pNGVL4a-E6/opt were delivered to the shaved abdominal regions of mice by using a helium-driven gene gun (Bio-Rad Laboratories Inc., Hercules, Calif.) with a discharge pressure of 400 lb/in^2. Mice were immunized with 2 lg of the DNA vaccine and received two boosts with the same dose at 1-week interval. Splenocytes were harvested 1 week after the last vaccination (Lin et al., 2006).
  • Challenge Protocol: One week after the last vaccination, mice were challenged with 5 x10^4 TC-1 tumor cells mouse subcutaneously in the right leg and monitored once a week by inspection and palpation (Lin et al., 2006).
  • Efficacy: C57BL/6 mice vaccinated with pNGVL4a-E6/opt are able to generate potent protective and therapeutic antitumor effects against challenge with E6-expressing tumor cell line, TC-1 of HPV (Lin et al., 2006).
References
Lin et al., 2006: Lin CT, Tsai YC, He L, Calizo R, Chou HH, Chang TC, Soong YK, Hung CF, Lai CH. A DNA vaccine encoding a codon-optimized human papillomavirus type 16 E6 gene enhances CTL response and anti-tumor activity. Journal of biomedical science. 2006; 13(4); 481-488. [PubMed: 16649071].