• Vaccine Name: H. pylori NAP protein vaccine
• Target Pathogen: Helicobacter pylori
• Target Disease: Ulcers
• Vaccine Ontology ID: VO_0011514
• Type: Subunit vaccine
• Status: Research
• Antigen: H. pylori neutrophil-activating protein
• NAP gene engineering:
  • Type: Recombinant protein preparation
  • Description: HP-NAP was cloned and expressed in Bacillus subtilis to avoid contamination with LPS. Two preparations of HP-NAP were isolated from H. pylori CCUG strain (Satin et al., 2000).
  • Detailed Gene Information: Click Here.
• Adjuvant: LTK63 vaccine mutant
  • Adjuvant name: LTK63 vaccine mutant
  • VO adjuvant ID: VO_0001251
  • Description: LTK63 mutant of Escherichia coli heat-labile enterotoxin (Satin et al., 2000)
• Immunization Route: Intragastric

Mouse Response

• Host Strain: CD-1
• Vaccination Protocol: Groups of 10 mice (Charles River) were immunized intragastrically at days 0, 7, and 14 with saline alone (control) or with saline containing 100 mg of H. pylori CagA, glutathione S-transferase (GST)–HP-NAP, or H. pylori lysate together with 10 mg of LTK63 mutant as a mucosal adjuvant (Satin et al., 2000).
• Challenge Protocol: At days 21, 23, and 25, all mice were challenged intragastrically with 10^9 CFU of H. pylori strain SPM326, a clinical isolate that has been adapted to colonize the mouse. At day 35, mice were killed, the stomachs were removed, and colonization was determined. Mice were considered as protected (not infected) when no H. pylori colony was detected on the stomach culture plates (Satin et al., 2000).
• Efficacy: Study shows that vaccination of mice with HP-NAP induces protection against H. pylori challenge, and that the majority of infected patients produce antibodies specific for HP-NAP, suggesting an important role of this factor in immunity (Satin et al., 2000).