• Vaccine Name: H. influenzae D15 protein vaccine
• Target Pathogen: Haemophilus influenzae
• Target Disease: Meningitis
• Vaccine Ontology ID: VO_0011551
• Type: Subunit vaccine
• Status: Research
• Antigen: H. influenzae protective surface antigen D15
• D15 gene engineering:
  • Type: Recombinant protein preparation
  • Description: Plasmid DNA for sequencing clones DS-712-2-1, DS-691-1-5, JB-1042-5-1, and JB-1042-9-4 was prepared from 50-ml overnight cultures with the Qiagen Plasmid Midi kit. DNA samples were sequenced on an ABI model 373A DNA sequencer with dye terminator chemistry. Plasmid JB-1042-5-1 contains the entire SB33 d15 gene (see Fig. 4). The ;2.9-kb HindIII-EcoRI d15 gene fragment was subcloned into pUC8-BgXb to generate plasmid pRY-60-1. Digestion of pRY- 60-1 with BsrFI and EcoRI excised a ;2.7-kb fragment containing most of the coding sequence of d15. The remainder of the DNA sequences coding for the amino terminus of D15 were recreated from ;90-bp NdeI-BsrFI oligonucleotides (Loosmore et al., 1997).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000133
• Immunization Route: Intramuscular injection (i.m.)

Rat Response

• Host Strain: Sprague- Dawley
• Vaccination Protocol: In the bacteremia models, Sprague-Dawley infant rats (Harlan Sprague Dawley, Indianapolis, Ind.) from three mothers were mixed and then randomly divided into three groups. In the type b model, groups of 12 to 13 5-day-old infant rats were inoculated s.c. on the dorsum close to the neck with 0.1 ml of rabbit anti-rD15 or anti-MinnA antiserum. The animals in the control group were injected with rabbit prebleed serum (Loosmore et al., 1997).
• Challenge Protocol: 24 hours after immunization, the animals were challenged intraperitoneally (i.p.) with 100 CFU of freshly grown Hib MinnA (0.1 ml) (Loosmore et al., 1997).
• Efficacy: Purified rD15 was found to be highly immunogenic in mice, guinea pigs, and rabbits, and passive transfer of anti-rD15 antibodies protected infant rats from challenge with H. influenzae type b or type a in infant rat models of bacteremia (Loosmore et al., 1997).