• Vaccine Name: P. yoelii DNA vaccine encoding PyHEP17 Protein
• Target Pathogen: Plasmodium spp.
• Target Disease: Malaria
• Vaccine Ontology ID: VO_0004162
• Type: DNA vaccine
• Status: Research
• HEP17 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005061
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/cByJ, A/J, B10.BR, B10.Q and C57BL/6
• Vaccination Protocol: Female 6- to 8-wk-old mice were immunized three times at 3-wk intervals intramuscularly in each tibialis anterior muscle with 50 μg of PyHEP17 DNA in 50 μl of saline or unmodified nkCMVintpolyli plasmid. 2 wk after the third immunization, mice were challenged by tail-vein injection with 100 infectious sporozoites or 200 infected erythrocytes (Doolan et al., 1996).
• Challenge Detail:

  No.	Pathogen Name	Dose	Route	Age	Interval
  1	Plasmodium yoelii	100 CFU in volume ml	Tail vein injection	day	14 day

• Efficacy Detail:

  No.	Efficacy method	Result	Description	Group Efficacy Detail
  1	In vivo protection study	CFU	Immunization with PyHEP17 DNA partially protected three of the five strains >20% against challenge with 100 infectious sporozoites. A 2 to 6 day delay in the onset of parasitemia in some nonprotected mice was consistent with partial immunity, which eliminated up to 90% of infected hepatocytes (Doolan et al., 1996).