• Vaccine Name: S. pneumoniae RASV synthesizing PsaA
• Target Pathogen: Streptococcus pneumoniae
• Target Disease: Pneumonia
• Vaccine Ontology ID: VO_0004071
• Type: Recombinant vector vaccine
• Status: Research
• psaA gene engineering:
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click Here.
• Immunization Route: Intranasally

Mouse Response

• Host Strain: BALB/c, C57BL/6J
• Vaccination Protocol: Mice were inoculated intranasally with 10 µl or orally with 20 µl of BSG containing 1 x 10^9 CFU of the RASV or control strain. In some experiments, the mice were boosted at week 6 with the same dose by using the same route as that used for primary immunization (Wang et al., 2010).
• Challenge Protocol: At week 10, mice were challenged either by intraperitoneal injection with 2 x 10^4 CFU of S. pneumoniae WU2 (equal to 100x the 50% lethal dose [LD50]) or intranasally with 20 µl containing 5 x 10^6 CFU S. pneumoniae strain L82016 or E134 or 1 x 10^7 CFU of strain A66.1 or D39 (Wang et al., 2010).
• Efficacy: BALB/c (haplotype H2(d)) or C57BL/6 (haplotype H2(b)) mice vaccinated either orally or intranasally exhibited a significant reduction in colonization of nasopharyngeal tissues after intranasal challenge with S. pneumoniae strains compared to controls, although protection was not observed with all challenge strains (Wang et al., 2010).