• Vaccine Name: S. pneumoniae ClpP protein Vaccine
• Target Pathogen: Streptococcus pneumoniae
• Target Disease: Pneumonia
• Vaccine Ontology ID: VO_0004006
• Type: Subunit vaccine
• Status: Research
• Antigen: ATP-dependent caseinolytic protease (ClpP)
• ClpP gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000127
  • Description: Imject Alum no. 77161
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Female BALB/c mice, weighing 16 to 18 g, were immunized three times at 14-day intervals with 10 µg of protein in alum adjuvant (3:1 [vol/vol]) of either ClpP protein in alum adjuvant or control protein in alum. Mice were boosted intraperitoneally with the same doses on days 14 and 28 (Wu et al., 2010).
• Challenge Protocol: For the intraperitoneal challenge models, at 2 weeks after the last immunization BALB/c mice were administrated the virulent pneumococcal strain D39 at 1,500 times the 50% lethal dose (LD50; 1.5 x 10^5 CFU) or 150 times the LD50 (2.0 x 10^4 CFU). In the focal pneumonia models, BALB/c mice were intranasally inoculated with pneumococcal strain 31614 (serotype 14, 1.5 x 10^6 CFU) or 31693 (serotype 19F, 10^7 CFU). For intranasal challenge with pneumococcal strain D39, mice inspired the virulent pneumococcal strain D39 at 104 (7.5 x 10^7 CFU) or 103 (6.0 x 10^6 CFU) times the LD50 in a volume of 40 µl under injection anesthesia (Wu et al., 2010).
• Efficacy: Compared to the control protein, ClpP reduced the median bacterial load nearly 100-fold in the focal pneumonia model (Wu et al., 2010).