• Vaccine Name: CTB-ESAT6-MPT64
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Rabbit
• Antigen: The antigen is composed of Mycobacterium tuberculosis antigens ESAT-6 and MPT-64 (Yadav et al., 2023).
• MPT64 gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Yadav et al., 2023).
  • Detailed Gene Information: Click Here.
• EsxA (ESAT-6) gene engineering:
  • Type: Fusion Protein
  • Description: It's use in prepare fusion protein (Yadav et al., 2023).
  • Detailed Gene Information: Click Here.
• Adjuvant: CTB vaccine adjuvant
  • Adjuvant name: CTB vaccine adjuvant
  • VO adjuvant ID: VO_0005587
  • Description: CTB vaccine adjuvant was used to generate ESAT6-MPT64 fusion protein vaccine (Yadav et al., 2023).
• Immunization Route: Mucosal (oral) immune route

Rabbit Response

• Vaccination Protocol: Rabbits were orally immunized by feeding 3 g of transgenic cucumber fruit expressing the CTB-ESAT-6-MPT-64 fusion protein on days 0, 7, and 14, with serum collected on day 21 for immunological analysis (Yadav et al., 2023).
• Immune Response: Oral administration of transgenic cucumber expressing CTB-ESAT-6-MPT-64 induced a significant increase in antigen-specific serum IgG levels compared with rabbits fed non-transgenic cucumber, demonstrating effective immunogenicity of the fusion protein (Yadav et al., 2023).