• Vaccine Name: T. cruzi Vaccine TcVac3 encoding TcG2 and TcG4
• Target Pathogen: Trypanosoma cruzi
• Target Disease: Chagas disease
• Type: DNA vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Antigen: TcG2 and TcG4 (Gupta and Garg, 2013) - T. cruzi encoding plasmids which have elicited a strong Th1 - type antibody response dominated by immunoglobin G2b (IgG2b)/IgG1 isotypes. (Bhatia and Garg, 2008)
• G2 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• TcG4 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001294
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001147
• Immunization Route: Intramuscular injection (i.m.)
• Description: DNA-prime/MVA-boost vaccine (TcVac3) constituted of antigenic candidates (TcG2 and TcG4) (Gupta and Garg, 2013)

Mouse Response

• Host Strain: C57BL/6
• Host age: 6-8 weeks old
• Host gender: Female
• Vaccination Protocol: Mice were injected with antigen-encoding plasmids with our without IL-12 and GM-CSF encoding plasmids. Three weeks later, mice were given booster vaccine constituted of rMVA.TcG2 and rMVA.TcG4. Mice injected with empty vectors were used as controls. (Gupta and Garg, 2013)
• Challenge Protocol: Two weeks after the last immunization, mice were challenged with T. cruzi. Mice were sacrificed at day 30 and 120-post infection corresponding to the acute phase of peak parasitemia and the chronic phase of disease development, respectively. (Gupta and Garg, 2013)