• Vaccine Name: PrV-PCV2-ORF2
• Target Pathogen: Porcine circovirus 2
• Target Disease: Postweaning multisystemic wasting syndrome
• Vaccine Ontology ID: VO_0004699
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• ORF2 gene engineering:
  • Type: Recombinant vector construction
  • Description: PCV2 ORF2 gene was inserted into vector pG to produce the recombinant PRV vector pGO; the genome of PRV attenuated vaccine and the transfer plasmid pGO were transfected by using Lipofectamine 2000 Reagent into swine testis cells for homologous recombination to obtain the recombinant PRV (Chao et al., 2014).
  • Detailed Gene Information: Click Here.
• Vector:
  • Vector name:
  • VO vector ID: VO_0001050
  • Description: (Chao et al., 2014)
• Preparation: PCV2 ORF2 gene was inserted into vector pG to produce the recombinant PRV vector pGO (Chao et al., 2014).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Six week odl mice were immunized two intramuscular immunizations 4 weeks apart (Chao et al., 2014).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: Mice were then challenged with the virulent PCV2 NY strain at 8 weeks after the first immunization (Chao et al., 2014).
• Efficacy: Challenge experiments show that the recombinant virus and PCV2 inactivated vaccine could both protect the mice against PCV2 challenge, suggesting that the recombinant virus can be an excellent potential vaccine (Chao et al., 2014).