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Vaccine Detail
RB51-SRS2 |
Vaccine Information |
- Vaccine Name: RB51-SRS2
- Target Pathogen: Neospora caninum
- Target Disease: Neosporosis
- Vaccine Ontology ID: VO_0004648
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- SRS2
gene engineering:
- Type: Recombinant vector construction
- Description: Recombinant RB51 strains each expressing SRS2 (RB51/SRS2) or GRA7 (RB51/GRA7) antigens of N. caninum (Alaeddine et al., 2005).
- Detailed Gene Information: Click Here.
- Vector:
- Preparation: RB51 strains expressing SRS2 (RB51/SRS2) or GRA7 (RB51/GRA7) antigens of N. caninum (Vemulapalli et al., 2007).
- Immunization Route: Intramuscular injection (i.m.)
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Host Response |
Mouse Response
- Vaccination Protocol: Mice were immunized by single intraperitoneal inoculation of the recombinant RB51 strains (Vemulapalli et al., 2007).
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: The vaccinated mice were challenged with N. caninum tachyzoites (Vemulapalli et al., 2007).
- Efficacy: Mice vaccinated with strain RB51/SRS2, but not RB51/GRA7, showed significant resistance to cerebral infection when compared to the RB51 vaccinated mice. Interestingly, mice vaccinated with either strain RB51 or RB51/GRA7 also contained significantly lower parasite burden in their brains compared to those inoculated with saline. Mice vaccinated with strain RB51/SRS2 or RB51/GRA7 were protected to the same extent as the strain RB51 vaccinated mice against challenge with B. abortus virulent strain 2308 (Vemulapalli et al., 2007).
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References |
Alaeddine et al., 2005: Alaeddine F, Keller N, Leepin A, Hemphill A. Reduced infection and protection from clinical signs of cerebral neosporosis in C57BL/6 mice vaccinated with recombinant microneme antigen NcMIC1. The Journal of parasitology. 2005; 91(3); 657-665. [PubMed: 16108562].
Vemulapalli et al., 2007: Vemulapalli R, Sanakkayala N, Gulani J, Schurig GG, Boyle SM, Lindsay DS, Sriranganathan N. Reduced cerebral infection of Neospora caninum in BALB/c mice vaccinated with recombinant Brucella abortus RB51 strains expressing N. caninum SRS2 and GRA7 proteins. Veterinary parasitology. 2007; 148(3-4); 219-230. [PubMed: 17651896].
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