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Vaccine Detail
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BCG-DHTM |
| Vaccine Information |
- Vaccine Name: BCG-DHTM
- Target Pathogen: Mycobacterium tuberculosis
- Target Disease: Tuberculosis
- Vaccine Ontology ID: VO_0004618
- Type: Recombinant vector vaccine
- Status: Research
- Antigen: BCG vaccine with deficient urease; a fusion protein composed of Mycobacterium tuberculosis-derived major membrane protein II (MMP-II) and heat shock protein 70 (HSP70) of BCG (Mukai et al., 2014).
- DnaK
gene engineering:
- Type: Recombinant vector construction
- Description: The M. tuberculosis gene dnaK, also known as hsp70, is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
- Detailed Gene Information: Click Here.
- bfrA
gene engineering:
- Type: Recombinant vector construction
- Description: The M. tuberculosis gene bfrA (also known as MMP-II) is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014).
- Detailed Gene Information: Click Here.
- Vector: BCG vaccine vector
- Immunization Route: subcutaneous injection
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| Host Response |
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Mouse Response
- Host Strain: C57BL/6J
- Host age: 5 weeks
- Vaccination Protocol: Five mice per group were vaccinated with BCG-261H (control) or BCG-DHTM at 1000 CFU/mouse for 6 weeks (Mukai et al., 2014)
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: Mice were challenged with H37RV at 100 CFU per lung by aerosol infection (Mukai et al., 2014)
- Efficacy: At 6 weeks post challenge, mice vaccinated with BCG-261H (control) or BCG-DHTM showed inhibited multiplication of M. tuberculosis in the lung, and BCG-DHTM inhibited M. tuberculosis multiplication more strongly than BCG-261H (Mukai et al., 2014).
- Information about this animal model: Mouse Model for TB research
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| References |
Mukai et al., 2014: Mukai T, Tsukamoto Y, Maeda Y, Tamura T, Makino M. Efficient activation of human T cells of both CD4 and CD8 subsets by urease-deficient recombinant Mycobacterium bovis BCG that produced a heat shock protein 70-M. tuberculosis-derived major membrane protein II fusion protein. Clinical and vaccine immunology : CVI. 2014; 21(1); 1-11. [PubMed: 24152387].
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