• Vaccine Name: Herpes Simplex Virus Type 1 DNA Vaccine encoding 5gP
• Target Pathogen: Herpes simplex virus type 1 and 2
• Target Disease: Herpes
• Vaccine Ontology ID: VO_0004520
• Type: DNA vaccine
• Status: Research
• UL27 from Herpes simplex virus type 1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• UL44 from HSV-1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• gD gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• US8 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• US7 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005075
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: The complete open reading frame for each of the five HSV-1 glycoproteins (gB, gC, gD, gE, and gI) was cloned into the VR-1055 expression vector and grown in bacteria. Plasmid DNA encoding each glycoprotein was purified on a cesium chloride gradient. In each experiment, 10 mice per group were inoculated intramuscularly (IM) into each quadriceps on days 0, 21, and 42 with a cocktail consisting of 10 μg of each cesium chloride–purified DNA (Osorio et al., 2004).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: All immunized groups had significantly higher neutralizing antibody titers than mock-vaccinated mice (Osorio et al., 2004).
• Challenge Protocol: Ocular challenge was performed 3 weeks after the final immunization. An inoculum of 2 × 105 or 2 × 106 pfu of HSV-1 strain McKrae in 5 μL tissue culture medium was placed in each eye without anesthesia and without corneal scarification, and the lid was gently rubbed for 30 seconds (Osorio et al., 2004).
• Efficacy: In all vaccine groups, 10 of 10 (100%) of the mice survived, whereas only 15 of 70 (21%) mock groups showed similar survival patterns after ocular infection (Osorio et al., 2004).