• Vaccine Name: Herpes Simplex Virus Type 1 DNA Vaccine pRSC-gD-IL-21
• Target Pathogen: Herpes simplex virus type 1 and 2
• Target Disease: Herpes
• Vaccine Ontology ID: VO_0004519
• Type: DNA vaccine
• Status: Research
• gD gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005076
• Immunization Route: Eye-drop immunization

Mouse Response

• Vaccination Protocol: For the vaccination experiments, Balb/c mice were randomly divided into pRSC-group (mock plasmid), pRSC-gD group, pRSC-gD-IL-21 group and pRSC-gD-IL-21+ nanoparticles group in order. 12 mice per group were used routinely. In the nanoparticles group, 20 μl (40 μg DNA) of the nanoparticles containing DNA vaccine pRSC-gD-IL-21 were topically dropped into the eye of each mouse on days 0, 14 and 28. In other groups, each mouse was inoculated topically into the eye with 40 μg DNA vaccine pRSC-gD-IL-21 or pRSC-gD or mock plasmid in 20 μl PBS 3 times at two week intervals (Hu et al., 2011).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: The nanoparticles containing DNA vaccine pRSC-gD-IL-21 induced mice to generate higher levels of specific neutralizing antibody, sIgA in tears, and IFN-γ, IL-4 in serum (Hu et al., 2011).
• Challenge Protocol: Three weeks after the final immunization, 6 mice per group were sacrificed and used for detecting immune efficiency, and the other 6 mice were used for HSV-1 challenge experiments. Mice were anesthetized by intraperitoneal injection of sodium pentobarbital (1.5 mg/0.15 ml) and the corneas of mice were scarified 10 times with 1 ml needle and the ocular mucosa of each eye were dropped with HSV-1 (10 μl of 5 × 106 plaque forming unit (PFU)/ml) (Hu et al., 2011).
• Efficacy: On day 10 after viral challenge, there was no keratitis in the mouse of the pRSC-gD-IL-21+ nanoparticles group, demonstrating that the pRSC-gD-IL-21+ nanoparticles vaccine was efficient to inhibit HSK in a mouse model (Hu et al., 2011).