• Vaccine Name: T. cruzi DNA vaccine pTS encoding T. cruzi antigens
• Target Pathogen: Trypanosoma cruzi
• Target Disease: Chagas disease
• Vaccine Ontology ID: VO_0004377
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• TS gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3 expressed T. cruzi antigens, including trans-sialidase (TS) (Eickhoff et al., 2011).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Vaccination of mice with pTS alone or pTS + pIL-15 elicited strong TS-specific T cell responses as detected 3 months after vaccination. In addition to enhancing TS-specific CD8+ T cell responses, the pIL-15 adjuvant enhanced TS-specific CD4+ T cell responses induced by pTS vaccination (Eickhoff et al., 2011).
• Efficacy: We challenged 5 mice per group with a lethal dose of T. cruzi BFT (5,000 BFT s.c.) 30 days following the final immunization, and followed survival for >2 months. All mice vaccinated with pTS (with or without pIL-15 co-immunization) survived lethal T. cruzi challenge, whereas all mice vaccinated with pIL-15 alone died (Eickhoff et al., 2011).