• Vaccine Name: Burkholderia pseudomallei aroC deletion mutant vaccine
• Target Pathogen: Burkholderia pseudomallei
• Target Disease: Melioidosis
• Vaccine Ontology ID: VO_0002825
• Type: Live, attenuated vaccine
• Status: Research
• aroC gene engineering:
  • Type: Gene mutation
  • Description:
  • Detailed Gene Information: Click Here.
• Preparation: An unmarked aroC deletion mutant of B. pseudomallei strain A2 was constructed using a sucrose counter-selection strategy. The aroC deletion in the resultant mutant, designated A2DeltaaroC, was confirmed by PCR, Southern hybridization and failure of the mutant to grow in a defined medium without aromatic compound (Srilunchang et al., 2009).
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Persistence: Compared to the parental wild type strain, A2DeltaaroC was highly attenuated for virulence following intraperitoneal introduction into BALB/c and C57BL/6 mice (Srilunchang et al., 2009).
• Efficacy: C57BL/6 mice immunized intraperitoneally with A2DeltaaroC were significantly protected against a challenge dose of 6,000 cfu (20 x LD50) (Srilunchang et al., 2009).