• Vaccine Name: H. pylori GltA Protein Vaccine
• Target Pathogen: Helicobacter pylori
• Target Disease: Ulcers
• Vaccine Ontology ID: VO_0004062
• Type: Subunit vaccine
• Status: Research
• Antigen: Purified GltA protein
• gltA gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000142
• Immunization Route: intra-Peyer's patch (IPP)

Mouse Response

• Host Strain: C57BL/6
• Vaccination Protocol: Mice were immunised by the intra-Peyer's patch (IPP) route with purified GltA (at 0.5 mg protein ml−1) was contained in an homogenate of equal quantities of PBS and Freund's incomplete adjuvant. For IPP immunisation each mouse was anaesthetised by intraperitoneal injection of 200 μl of a ketamine, xylazine mixture made by mixing 10 ml of ketamine (100 μg ml−1) and 1 ml of xylazine (100 μg ml−1), the abdomen shaved and swabbed with 70% alcohol and a midline incision made in the skin and muscle layers to expose the intestine. Visible Peyer's patches were located along the length of the intestine and approximately 3 μl of homogenate injected directly under the serosa of each Peyer's patch (Dunkley et al., 1999).
• Challenge Protocol: Mice were infected 2 weeks after immunisation with H. pylori Sydney strain 1 (SS1) (Dunkley et al., 1999).
• Efficacy: Mice were immunised with the citrate synthase (GltA) protein by intra-Peyer's patch immunisation. Pre-immunisation with the citrate synthase protein led to a 84-91% reduction in H. pylori infection compared to unimmunised controls (Dunkley et al., 1999).