• Vaccine Name: L. major PSA-2 Protein Vaccine
• Target Pathogen: Leishmania major
• Target Disease: Cutaneous leishmaniasis
• Vaccine Ontology ID: VO_0004044
• Type: Subunit vaccine
• Status: Research
• Host Species as Laboratory Animal Model: mouse
• PSA-2 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001259
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: C3H/He and BALB/c H-2^k
• Vaccination Protocol: Groups of 8 to 16 mice were injected three times, every 2 weeks, intraperitoneally with 1.5 to 2 mg of purified PSA-2mixed with 200 mg of killed Corynebacterium parvum as an adjuvant (Handman et al., 1995).
• Challenge Protocol: Two weeks after the last injection, all mice were bled individually and divided into groups; one group was used to examine T-cell responses to PSA-2 antigen, and another group was challenged with 10^5 live promastigotes.
• Efficacy: Intraperitoneal vaccination of C3H/He mice with PSA-2 with Corynebacterium parvum as an adjuvant resulted in complete protection from lesion development after challenge infection with virulent L. major. Significant protection was also obtained in the genetically susceptible BALB/cH-2k and BALB/c mice (Handman et al., 1995).