• Vaccine Ontology ID: VO_0011419
• Type: Subunit vaccine
• Status: Research
• HN gene engineering:
  • Type: Recombinant protein preparation
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • VO ID: VO_0000127
• Immunization Route: Intramuscular injection (i.m.)

Monkey Response

• Host Strain: Rhesus macaques
• Vaccination Protocol: Six monkeys in Group 1 were immunized with 10 μg purified vaccine and adjuvant (aluminum hydroxide, aluminum content: 1.0 mg/ml). Six monkeys in Group 2 were immunized with 20 μg purified vaccine and adjuvant. Two monkeys in Group 3 were immunized with attenuated vaccine (4.1 log CCID50). Two monkeys in Group 4 were mock immunized with 0.9% saline. All experimental groups were immunized by deltoid muscle injection and boosted once per 4-week interval (Liang et al., 2008).
• Challenge Protocol: Four weeks after the second immunization, all four monkeys in groups of experimental vaccine and all two monkeys in group of attenuated vaccine were challenged with virus, while the others remained uninfected to test their antibody titers at 2, 3, 4 and 6 months after the second dose (Liang et al., 2008).
• Efficacy: At 1 week after the challenge injection, neutralization antibody was induced in the immunized monkeys to a higher level than in the control monkeys. The clinical and pathological observations after challenge of vaccinated rhesus monkeys indicated further that the immune response induced by this vaccine provided complete protection from wild-type virus infection (Liang et al., 2008).