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PotF
Gene Name	PotF
Sequence Strain (Species/Organism)	Burkholderia pseudomallei K96243
VO ID	VO_0010922
NCBI Gene ID	3095748
NCBI Protein GI	53721504
Locus Tag	BPSS0467
Genbank Accession	BX571966
Protein Accession	YP_110489
Taxonomy ID	272560
Chromosome No	2
Gene Starting Position	636534
Gene Ending Position	637655
Gene Strand (Orientation)	-
Protein Name	ABC transporter substrate-binding protein
Protein pI	6.89
Protein Weight	37606.43
Protein Length	373
Protein Note	Similar to Escherichia coli putrescine-binding periplasmic protein precursor PotF or b0854 SWALL:POTF_ECOLI (SWALL:P31133) (370 aa) fasta scores: E(): 3.3e-67, 49.3% id in 357 aa, and to Pseudomonas aeruginosa polyamine transport protein pa0301 SWALL:Q9I6J0 (EMBL: AE004468) (365 aa) fasta scores: E(): 6.3e-69, 49.59% id in 367 aa. Possible alternative translational start site
DNA Sequence	>NC_006351.1:636534-637655 Burkholderia pseudomallei K96243 chromosome 2, complete sequence ATCAGTGTCCCGTCTTCAATTGCGCCCACAGACGGTTCTCGAGCCGCAGGATGTCGGCGGGCATCGGCCG CATCAGCGTCATCTTCTTGAGCACGTCCTCGGGCGGGTAGACGGTCGGGTCCTGCGCGACGGCGGGCACG ACGAACTGTCGCGCCGCGCGGTTCGCGCTCGGGTAGAACACTTCGTTGGTGATCGCCGCGTTGACCTTCG GGTCCTCGATGTAGTTGATCCACTTCAGCGCGGCTTCCGCGTGCGGCGCATCCTTCGGGATCACCATCAC GTCGAACCACAGCAGGCCGCCTTCCTTCGGATTCGAGAACTTGATGTCGTACGGGCGCTTCGCCTCGGCC GAGCGGCGATGCGCGATGCCGACGTCGCCCGACCAGCCGAGCGCGACGCATACGTCGTTGTTCGCGAGAT CGTTGATGTAGCCGGACGAGTTGAATTGCGTGATGTACGGGCGGACTTTCTTCAGCACCTCGAACGCCGC CTGGTAATCGGCCGGGTTCGTGCTGTTCGGATTCCTGCCCATGTATTGTAGCGTCGCGGCGAACACGTCG ACCGCCTGATCGAGGAACGATACGCCGCAGCCCTTGAGCTTGGATACGTTCGCCGGATCGAGCACGAGCG CCCAGCTGTCGACGGGCGCGTTCTCGCCGAGCGCCTTCTTCACCGCCTGCACGTTGTAGCCGAGGCCGTC GGTGCCGAACGCCCACGGCACGCCGTACTGGTTGCCCGGATCGGCGTCGGCGATCATCTTCATCAGCGTC GGATCGAGGTTCGACAGGTTCGGCAGCTTCGATTTGTCGAGCTTCTGGTAGACGCCCGCCTGAATCTGCT TGGCCATGTAGTTCGACGTCGGCACGACGATGTCGTAGCCGGAGCTGCCCGCGAGCAGCTTCGCCTGCAG CGTGTCGTCGCTGTCGTAGTTGTCGTACTTGACGTGGATGCCGGATTGCTTCTCGAAGTTCGGGATCGTG TCCTTCGCGATGTAGTCCGACCAGTTGTACACGTTCAGCTCGGCGCCGGCCGCGTGCGCGGCCGGCAGGG CGAGCGCCGACGCGCCGGCGAGCGCGACGAGCGCTGCGCGCGCGACCGCATGACGAAGATGGCTCACACT CA
Protein Sequence	>YP_110489.1 ABC transporter substrate-binding protein [Burkholderia pseudomallei K96243] MSVSHLRHAVARAALVALAGASALALPAAHAAGAELNVYNWSDYIAKDTIPNFEKQSGIHVKYDNYDSDD TLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLSNLDPTLMKMIADADPGNQYGVPWAFG TDGLGYNVQAVKKALGENAPVDSWALVLDPANVSKLKGCGVSFLDQAVDVFAATLQYMGRNPNSTNPADY QAAFEVLKKVRPYITQFNSSGYINDLANNDVCVALGWSGDVGIAHRRSAEAKRPYDIKFSNPKEGGLLWF DVMVIPKDAPHAEAALKWINYIEDPKVNAAITNEVFYPSANRAARQFVVPAVAQDPTVYPPEDVLKKMTL MRPMPADILRLENRLWAQLKTGH
Molecule Role	Protective antigen
Molecule Role Annotation	PotF was evaluated as an antigen for a vaccine candidate against B. pseudomallei infection. Nonmembrane regions of the B. pseudomallei protein was expressed and purified from Escherichia coli and then evaluated as a vaccine candidate in an established mouse model of B. pseudomallei infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the protein stimulated antigen-specific humoral and cellular immune responses. Immunization with the PotF protein domain afforded significant protection against a subsequent challenge with B. pseudomallei (Harland et al., 2007).
Related Vaccines(s)	B. pseudomallei Subunit PotF Protein Vaccine
References

PotF