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SecA2
Gene Name	SecA2
Sequence Strain (Species/Organism)	Mycobacterium tuberculosis H37Rv
VO ID	VO_0010950
NCBI Gene ID	885594
NCBI Protein GI	15608958
Locus Tag	Rv1821
Genbank Accession	BX842577
Protein Accession	NP_216337
3D structure: PDB ID	1NL3
Other Database IDs	EMBL: AE000516 EMBL: BX842577 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR HSSP: O05885 InterPro: IPR000185 InterPro: IPR001650 InterPro: IPR011115 InterPro: IPR011116 InterPro: IPR011130 InterPro: IPR014018 InterPro: IPR014021 KEGG: mtc:MT1869 KEGG: mtu:Rv1821 Pfam: PF01043 Pfam: PF07516 Pfam: PF07517 PIR: F70720 PRINTS: PR00906 PROSITE: PS01312 PROSITE: PS51196 TIGR: MT1869 TubercuList: Rv1821 UniProt: P66785
Taxonomy ID	83332
Gene Starting Position	2066456
Gene Ending Position	2068882
Gene Strand (Orientation)	+
Protein Name	preprotein translocase subunit SecA
Protein pI	5.11
Protein Weight	82502.57
Protein Length	808
Protein Note	SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins
Protein Annotation	FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785) SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785) SUBCELLULAR LOCATION: Cell membrane; peripheral membrane protein; cytoplasmic side (By similarity) (UniProt: P66785) SIMILARITY: Belongs to the secA family (UniProt: P66785)
DNA Sequence	>gi\|57116681:2066456-2068882 Mycobacterium tuberculosis H37Rv, complete genome CGTGAACGTGCACGGTTGTCCACGAATTGCGGCCTGTCGGTGTACAGACACGCACCCTCGCGGCCGGCCG GCATTCGCGTACCGTTGGTTTGTGCCCAAGACCACCCGCGCTCAACCCGGCCGGCTGAGCAGCCGATTCT GGCGATTGCTCGGCGCCAGCACCGAAAAGAACCGGAGCCGCTCCCTGGCGGATGTAACCGCTTCGGCAGA ATACGACAAGGAAGCTGCCGATCTGTCCGACGAGAAGCTGCGTAAGGCGGCAGGCCTGCTCAACCTCGAC GACCTCGCGGAGTCCGCCGATATCCCGCAGTTTCTCGCGATTGCCCGGGAAGCCGCCGAGCGGAGGACCG GGCTGCGACCATTTGATGTGCAGTTGCTTGGCGCGTTGCGCATGCTCGCCGGAGACGTGATCGAGATGGC CACCGGTGAGGGCAAAACCCTTGCCGGGGCGATCGCGGCCGCCGGTTATGCGCTGGCCGGCCGGCACGTG CACGTCGTGACGATTAACGATTACCTGGCCCGCCGCGATGCGGAGTGGATGGGCCCGCTGCTGGACGCGA TGGGCCTGACGGTCGGCTGGATCACCGCGGACTCGACCCCTGACGAGCGCCGGACCGCATATGACCGTGA TGTCACCTATGCCTCGGTCAACGAGATTGGCTTCGATGTACTGCGCGATCAGTTGGTGACTGATGTCAAT GACCTGGTATCGCCCAATCCAGACGTGGCTCTCATCGACGAAGCCGACTCCGTGCTGGTCGACGAGGCGC TGGTGCCCCTGGTGCTGGCCGGAACCACACATCGTGAGACGCCGCGGCTGGAGATCATCCGGCTGGTCGC TGAGCTTGTTGGCGACAAGGACGCCGACGAGTACTTTGCCACCGATTCCGATAACCGCAATGTCCACTTG ACCGAGCACGGGGCACGCAAAGTCGAGAAAGCGCTCGGTGGCATCGACCTGTACTCCGAGGAGCACGTCG GCACCACACTGACTGAGGTCAATGTCGCGCTGCACGCGCATGTGCTCCTGCAACGCGACGTGCACTACAT CGTCCGCGACGACGCGGTGCACCTGATCAACGCGTCGCGTGGCCGTATCGCGCAACTGCAGCGCTGGCCG GACGGGTTGCAAGCTGCGGTCGAGGCCAAGGAAGGTATCGAGACCACGGAAACTGGGGAAGTGCTCGACA CCATCACGGTGCAGGCCCTGATCAACCGGTATGCGACTGTGTGCGGAATGACGGGAACCGCGCTGGCCGC CGGTGAGCAGCTACGGCAGTTCTACCAGCTCGGTGTCTCACCGATACCACCGAACAAGCCAAACATCCGC GAGGACGAGGCCGACCGGGTCTACATCACCACTGCAGCCAAGAACGACGGGATCGTCGAGCACATCACCG AGGTGCACCAGAGGGGGCAGCCTGTGCTGGTCGGTACCCGCGACGTGGCCGAATCCGAGGAACTGCACGA ACGCCTGGTGCGCCGCGGTGTGCCCGCCGTGGTGCTCAACGCGAAGAACGACGCCGAGGAGGCCCGGGTC ATCGCCGAGGCCGGCAAATACGGCGCGGTCACGGTGTCAACTCAAATGGCCGGGCGCGGCACCGACATCA GGCTCGGCGGGTCCGACGAAGCTGACCACGACAGGGTCGCGGAATTGGGCGGCCTGCACGTGGTCGGCAC TGGCCGTCACCACACCGAGCGGCTAGACAACCAGCTGCGCGGTCGGGCCGGGCGCCAGGGAGATCCCGGG TCGTCGGTGTTTTTCTCAAGCTGGGAAGACGATGTCGTTGCGGCCAACCTCGACCACAACAAGCTGCCGA TGGCAACCGACGAAAATGGCCGGATTGTCAGCCCGAGGACGGGTAGTCTGCTCGACCATGCCCAGCGCGT TGCCGAGGGCCGGTTATTGGATGTGCACGCCAACACGTGGCGCTACAACCAGCTGATCGCCCAGCAGCGC GCCATCATCGTCGAACGGCGTAACACGTTGTTGCGCACCGTAACCGCGCGTGAGGAACTCGCCGAACTGG CGCCTAAGCGGTACGAGGAGCTGTCCGACAAAGTATCCGAGGAACGCCTCGAGACGATTTGTCGGCAGAT CATGCTGTATCACCTCGACCGTGGCTGGGCCGATCACCTGGCGTATCTGGCCGACATCCGGGAGAGCATC CATCTACGCGCGCTGGGCCGGCAGAACCCACTCGACGAGTTTCACCGGATGGCTGTGGACGCGTTCGCGT CGCTGGCCGCCGACGCCATCGAGGCGGCTCAACAGACGTTCGAAACCGCGAACGTCCTTGACCACGAGCC GGGGCTGGACCTGTCCAAACTGGCCCGGCCGACGTCGACATGGACCTACATGGTCAATGACAACCCACTG TCCGATGACACGCTTTCTGCCCTCAGTCTGCCCGGGGTGTTCCGCTG
Protein Sequence	>gi\|15608958\|ref\|NP_216337.1\| preprotein translocase subunit SecA [Mycobacterium tuberculosis H37Rv] MNVHGCPRIAACRCTDTHPRGRPAFAYRWFVPKTTRAQPGRLSSRFWRLLGASTEKNRSRSLADVTASAE YDKEAADLSDEKLRKAAGLLNLDDLAESADIPQFLAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMA TGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRD VTYASVNEIGFDVLRDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEIIRLVA ELVGDKDADEYFATDSDNRNVHLTEHGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYI VRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAA GEQLRQFYQLGVSPIPPNKPNIREDEADRVYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHE RLVRRGVPAVVLNAKNDAEEARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEADHDRVAELGGLHVVGT GRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVVAANLDHNKLPMATDENGRIVSPRTGSLLDHAQRV AEGRLLDVHANTWRYNQLIAQQRAIIVERRNTLLRTVTAREELAELAPKRYEELSDKVSEERLETICRQI MLYHLDRGWADHLAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDHEP GLDLSKLARPTSTWTYMVNDNPLSDDTLSALSLPGVFR
Molecule Role	Virmugen
Molecule Role Annotation	Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination (Hinchey et al., 2007). In another study, lysine auxotrophy combined with deletion of the SecA2 gene made a live attenuated vaccine for Mycobacterium tuberculosis. SCID mice was highly protected from challenge after the vaccination (Hinchey et al., 2011).
Related Vaccines(s)	M. tuberculosis secA2 mutant , Mycobacterium tuberculosis lysA/secA2 mutant vaccine
References

SecA2