• Vaccine Name: M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0011478
• Type: Other
• Status: Research
• Apa gene engineering:
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005030
• Immunization Route: Intradermal injection (i.d.)

Guinea pig Response

• Vaccination Protocol: Animals were immunized intradermally with APADNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by APAMVA, 10^7 PFU, given 4 weeks after the third DNA (at 12 weeks); or BCG Guindy, 10^6 CFU, given one time only (at week 0) or control DNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by MVA wild type, 10^7 PFU, given 4 weeks after the third DNA (at week 12) (Kumar et al., 2003).
• Challenge Protocol: Animals were challenged intramuscularly in the thigh muscle at 4 weeks after the last immunization with 2 × 10^5 viable M. tuberculosis strain NTI64719. This challenge dose was sufficient to cause measurable clinical illness in all control animals within a relatively short time frame of 6 weeks (Kumar et al., 2003).
• Efficacy: A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003).