• Vaccine Name: N. miningitidis TBP2 Protein Vaccine
• Target Pathogen: Neisseria meningitidis
• Target Disease: Meningitis
• Vaccine Ontology ID: VO_0004014
• Type: Subunit vaccine
• Status: Research
• tbp2 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000127
• Immunization Route: Subcutaneous Injection

Mouse Response

• Vaccination Protocol: Groups of 48 mice were immunized subcutaneously on days 0, 21, and 35 with different vaccine preparations containing 5 mg of protein adsorbed onto 0.1 mg of aluminum hydroxide per 0.5 ml; PBS containing aluminum hydroxide and heat-inactivated N. meningitidis B16B6 were used as controls (Lissolo et al., 1995).
• Challenge Protocol: 41 days after immunization, mice within a group were subdivided into groups of eight, and each subgroup received by the intraperitoneal route 24 mg of iron-loaded hTf (Sigma) and, immediately after by the intraperitoneal route, 0.5 ml of N. meningitidis grown under iron restriction. The bacterial load varied from 10^4 to 10^8 CFU. Mortality rates were measured for 5 days following challenge (Lissolo et al., 1995).
• Efficacy: Mice immunized with purified Tbp2 survived a lethal challenge to a similar degree as animals immunized with the Tbp1-Tbp2 complex, demonstrating that Tbp2 played an important role in the protective activity observed with the complex, eliciting antibodies that are not only bactericidal but also inhibitory for meningococcal growth (Lissolo et al., 1995).