• Vaccine Name: N. meningitidis ExbB Protein Vaccine
• Target Pathogen: Neisseria meningitidis
• Target Disease: Meningitis
• Vaccine Ontology ID: VO_0004011
• Type: Subunit vaccine
• Status: Research
• ExbB gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000142
• Immunization Route: Subcutaneous injection

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: To examine the protective efficacy of recombinant proteins, adult mice (6-week-old, female BALB/c animals, 35 per group) were immunised on days 1 and 21 with 25 μg of antigen given by subcutaneous (s.c.) injection. Each protein was mixed with Freund's incomplete adjuvant (50% v/v) prior to administration. Mice in control groups were immunised with adjuvant alone or recombinant homologous PorA prior to challenge (Sun et al., 2005).
• Challenge Protocol: 28 days after immunization, animals were challenged with live bacteria at either a high (10^7 CFU, 15 mice) or low (10^6 CFU, 15 mice) dose of MC58 (Sun et al., 2005).
• Efficacy: ExbB, which is required for iron acquisition, elicited protective immunity and was able to protect mice from bacterial challenge (Sun et al., 2005).