• Vaccine Name: N. caninum MIC10 and p24 protein vaccine
• Target Pathogen: Neospora caninum
• Target Disease: Neosporosis
• Vaccine Ontology ID: VO_0004164
• Type: Subunit vaccine
• Status: Research
• MIC10 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001266
• Immunization Route: Subcutaneous injection

Mouse Response

• Host Strain: Qs
• Vaccination Protocol: Briefly female Qs mice at 4–5 weeks of age were divided into groups that were injected subcutaneously (s.c.). VSA-3 was used as adjuvant (comprising 1/3 v/v of the immunogen) and 10 μg of each recombinant protein or lysate was injected per mouse. Four weeks later the same mice were given a booster s.c. injection of the same formulation received earlier (Ellis et al., 2008).
• Challenge Protocol: On day 5 of gestation, pregnant mice in the treatment groups were injected s.c with 10^6 tachyzoites of NC-Liverpool recovered from in vitro culture as described above. A control group (pregnant uninfected mice) were injected s.c. with 0.9% saline. On approx. day 14 of gestation pregnant dams were placed in individual boxes and allowed to carry their pregnancy to term. Mice were checked daily until all that were obviously pregnant had given birth and the date of birth and number of pups (live and dead) noted (Ellis et al., 2008).
• Efficacy: A mixture of MIC10 and p24B produced partial protection against transplacental transmission of N. caninum in this mouse model (Ellis et al., 2008).