• Vaccine Name: E. coli C3389 protein vaccine
• Target Pathogen: Escherichia coli
• Target Disease: Hemorrhagic colitis
• Vaccine Ontology ID: VO_0011417
• Type: Subunit vaccine
• Status: Research
• Antigen: E. coli C3389
• ompA gene engineering:
  • Type: Recombinant protein preparation
  • Description: The chromosomal DNA of 29 E. coli strains belonging to various phylogenetic groups was prepared using a standard molecular biology protocol (Promega). The membranes were hybridized with [α-33P]dCTP-radiolabeled DNA (Amersham Pharmacia Biotech, United Kingdom) overnight under stringent conditions (Durant et al., 2007).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000133
• Immunization Route: Subcutaneous injection

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Purified recombinant proteins were used to immunize groups of 6-week-old BALB/c@Rj mice (Janvier Laboratories, France). Each mouse was injected subcutaneously with 20 μg of recombinant protein emulsified in complete Freund's adjuvant (Sigma) on day 1. Three weeks later (day 21), the mice were given a boosting injection with 10 μg of recombinant protein emulsified in incomplete Freund's adjuvant. A control group was included in each experiment that consisted of mice injected on days 1 and 21 with PBS and adjuvant alone (Durant et al., 2007).
• Challenge Protocol: Control and immunized groups of mice were challenged on day 42 by intraperitoneal injection of E. coli S26 at a dose that caused death in 50% of the mouse population (LD50) (5 × 10^5 CFU/mouse). The survival of mice was monitored for 2 days after challenge. The survival rate in the vaccinated group was compared to the one obtained in the control group (Durant et al., 2007).
• Efficacy: Active immunization of BALB/c mice with recombinant E. coli protein C3389 antigen in Freund's adjuvant protects mice from lethal challenge with ExPEC strain S26 (Durant et al., 2007).