• Vaccine Name: E. tenella GX3262 protein vaccine
• Target Pathogen: Eimeria tenella
• Target Disease: Hemorrhagic cecal coccidiosis
• Vaccine Ontology ID: VO_0011470
• Type: Subunit vaccine
• Status: Research
• Antigen: E. tenella sporozoite antigen GX3262
• GX3262 gene engineering:
  • Type: Recombinant protein preparation
  • Description: To produce the 3-galactosidase-GX3262 fusion protein (Pgal-GX3262), recombinant E. coli cells. grown overnight in Luria-Bertani broth with ampicillin and induced with IPTG, were lysed by sonication and centrifuged, and the insoluble cell pellet was washed extensively with PBS (Miller et al., 1989).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
• Immunization Route: Subcutaneous injection

Chicken Response

• Host Strain: Hubbard broiler
• Vaccination Protocol: The basic experimental design involved immunizing birds subcutaneously or orally with partially purified recombinant antigen preparations, heatkilled bacterins. live E. coli cells, or control materials (Miller et al., 1989).
• Challenge Protocol: Broilers were challenged with E. tendell/i oocysts; and then evaluated for cecal lesions (Miller et al., 1989).
• Efficacy: A cDNA clone derived from sporulated oocysts of Eimeria tenella and encoding the expression product GX3262 was identified using a monoclonal antibody raised against Eimeria acervulina sporozoites. After challenge with an experimental E. tenella infection, the greatest degree of protection in immunized broiler chickens was seen after only a single immunization of 2-day-old birds with a live recombinant E. coli preparation (Miller et al., 1989).