• Vaccine Name: Y. pestis DNA vaccine encoding dfF1 Protein
• Target Pathogen: Yersinia pestis
• Target Disease: Plague
• Vaccine Ontology ID: VO_0004158
• Type: DNA vaccine
• Status: Research
• Antigen: F1 devoid of its putative signal peptide (deF1) (Grosfeld et al., 2003).
• caf1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000212
• Immunization Route: Gene Gun

Mouse Response

• Host Strain: ICR, BALB/c and DBA/2
• Vaccination Protocol: For gene gun immunization, plasmid DNA was precipitated onto 1-μm-diameter gold particles at a ratio of 2 μg per milligram of gold and loaded onto Gold-Coat tubing as suggested by the manufacturer (Bio-Rad). Polyvinylpyrrolidone (MW, 360,000) was used as an adhesive at a concentration of 0.05 mg/ml. Agarose gel electrophoresis was used to determine the amount of DNA. Vaccination was carried out with three immunizations of 0.5 μg of DNA at 2-week intervals. Gene gun shots were directed into exposed abdominal dermis (Grosfeld et al., 2003).
• Challenge Protocol: Immunized mice were challenged by s.c. injection of the indicated amounts of Y. pestis Kimberley53 strain suspension (0.1 ml). Animals were monitored daily for survival for a period of 3 weeks (Grosfeld et al., 2003).
• Efficacy: Immunized mice all survived lethal challenge with Y. pestis (Grosfeld et al., 2003).