• Vaccine Name: E. maxima Gam82 protein vaccine
• Target Pathogen: Eimeria maxima
• Target Disease: Coccidiosis
• Vaccine Ontology ID: VO_0011467
• Type: Subunit vaccine
• Status: Research
• Antigen: E. maxima 82 kDa gametocyte antigen (Gam82)
• gam82 gene engineering:
  • Type: Recombinant protein preparation
  • Description: The Gam82 coding sequence was amplified by PCR using Proof Start DNA polymerase (Qiagen, Valencia, CA). Amplicons were cloned into the pET28a (+) plasmid vector (Novagen/EMD Chemicals, Gibbstown, NJ) downstream from an NH2-terminal His6 epitope tag, plasmid clones were verified by sequence analysis, and used to transform competent Escherichia coli BL21 Star (Invitrogen). Eluted protein fractions were visualized on 10% SDS-acrylamide gels stained with Coomassie brilliant blue and on Western blots probed with horseradish peroxidase-conjugated anti-His monoclonal antibody (1:3000; Qiagen), and stored at −20 °C (Jang et al., 2010).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
  • Description: Freund's complete adjuvant (FCA)
• Immunization Route: Intramuscular injection (i.m.)

Chicken Response

• Host Strain: Ross broiler
• Vaccination Protocol: At 1 wk of age, chickens (30/group) were intramuscularly immunized with 100 μl of 300 or 600 μg/ml of recombinant Gam82 in Freund's complete adjuvant (FCA). Control animals received PBS in FCA. At 1 wk post-immunization, animals were given a booster injection with 100 μl of 300 or 600 μg/ml of recombinant Gam82, 3-1E, or PBS in Freund's incomplete adjuvant Ref1077:Jang et al., 2010].
• Challenge Protocol: At 7 d post-secondary immunization, animals were given PBS or 2.0 × 10^4 E. maxima sporulated oocysts by oral gavage using an 18-gauge needle (Jang et al., 2010).
• Efficacy: Animals vaccinated with Gam82 and challenged with E. maxima showed lower oocyst shedding and reduced intestinal pathology compared with non-vaccinated and parasite-challenged animals. Gam82 vaccination also stimulated the production of antigen-specific serum antibodies and induced greater levels of IL-2 and IL-15 mRNAs compared with non-vaccinated controls. These results demonstrate that the Gam82 recombinant protein protects against E. maxima and augments humoral and cell-mediated immunity (Jang et al., 2010).
• Host IL15 response
  • Description: Three and six days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-15 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010).
  • Detailed Gene Information: Click Here.
• Host IL2 response
  • Description: Three days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-2 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010).
  • Detailed Gene Information: Click Here.