• Vaccine Name: T. gondii DNA vaccine pcDNA3-MIC3
• Target Pathogen: Toxoplasma gondii
• Target Disease: Toxoplasmosis
• Vaccine Ontology ID: VO_0011523
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: mouse
• MIC3 gene engineering:
  • Type: DNA vaccine construction
  • Description: Protein MIC3 (Xiang et al., 2009).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Footpad injection

Mouse Response

• Host Strain: Kunming
• Vaccination Protocol: All studies were done with 6–8 weeks old Kunming mice. For experimental group, 100 μg pcDNA3-MIC3 were injected into mouse hind footpad three times, at week 0, 3 and 6, respectively. The mice of control groups were vaccinated with 100 μg pcDNA3 or normal saline (NS) three times (Xiang et al., 2009).
• Challenge Protocol: Immunized Kunming mice were challenged intraperitoneally with 500 tachyzoites of RH strain T. gondii 9 weeks after the first immunization (Xiang et al., 2009).
• Efficacy: The survival time of mice in pcDNA3-MIC3 group was significantly longer than those of in group pcDNA3 and NS (Xiang et al., 2009).