• Vaccine Name: Dengue DNA Vaccine encoding NS1 Protein
• Target Pathogen: Dengue Virus
• Target Disease: Dengue Fever
• Vaccine Ontology ID: VO_0011454
• Type: DNA vaccine
• Status: Research
• NS1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000132
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: C57/BL6, Balb/c, and C3H
• Vaccination Protocol: Groups of 3–4 weeks old female C3H mice were initially immunized with 80 μg of recombinant DNA pD2NS1, or pcDNA3 as a control. Recombinant pD2NS1 in PBS, control pcDNA3 in PBS, or PBS buffer alone was intramuscularly (i.m.) injected into the mouse thighs; the mice were subsequently boosted twice using the same method at 1 week interval (Wu et al., 2003).
• Challenge Protocol: One week after the final boost, mice were i.v. challenged with 5×10^6 plaque-forming units of PL046 in 100 μl PBS. The mice after virus challenge were monitored daily for morbidity and mortality for weeks (Wu et al., 2003).
• Efficacy: Intravenously challenged by lethal DEN-2, mice vaccinated with NS1-DNA exhibited a delay onset of paralysis, a marked decrease of morbidity, and a significant enhancement of survival (Wu et al., 2003).