• Vaccine Name: C. posadasii Subunit GEL1 Protein Vaccine
• Target Pathogen: Coccidioides spp.
• Target Disease: Coccidioidomycosis (California disease, Desert rheumatism, San Joaquin valley fever, and Valley fev
• Vaccine Ontology ID: VO_0011490
• Type: Subunit vaccine
• Status: Research
• GEL1 gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001237
  • Description: CpG ODN (Integrated DNA Technologies, Inc., Coralville, Iowa) (Delgado et al., 2003).
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c, C57BL/6
• Vaccination Protocol: Mice were immunized subcutaneously (s.c.) with rGel1p plus adjuvant. The mice were then boosted by s.c. immunization 14 days later with the same amount of immunogen plus adjuvant (Delgado et al., 2003).
• Challenge Protocol: The animals were subsequently challenged with 100 viable arthroconidia by the i.p. route 2 weeks after the last immunization and then sacrificed 12 days later to determine the residual CFU in the lungs and spleen (Delgado et al., 2003).
• Efficacy: rGel1p-immune mice infected with the pathogen showed a significant reduction in fungal burden and increased survival compared to nonimmune mice (Delgado et al., 2003).