• Vaccine Name: C. jejuni DNA vaccine pcDNA3.1(+)-peblA
• Target Pathogen: Campylobacter jejuni
• Target Disease: Campylobacterosis
• Vaccine Ontology ID: VO_0011488
• Type: DNA vaccine
• Status: Research
• Antigen: C. jejuni bifunctional adhesin/ABC transporter aspartate/glutamate-binding protein
• peb1A gene engineering:
  • Type: DNA vaccine construction
  • Description: The chitosan-DNA vaccine was prepared by embedding pcDNA3.1(+)-peblA with chitosan (Zheng et al., 2007).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intranasal

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: BALB/c mice were intranasally immunized in a four-dose primary series (7 d intervals) at doses of 60 microg chitosan-DNA vaccines each time (Zheng et al., 2007).
• Immune Response: The mice immunized with chitosan-DNA vaccines have generated high levels of IgA and IgG from the sera and IgA from the intestinal secretions and the P/N value went up to 20.58, 30.13 and 6.87, respectively (Zheng et al., 2007).
• Challenge Protocol: Mice were attacked repeatedly through intragastric administration of C. jejuni HS:19 at the 8th week after the immunization and protective efficacy was determined by detecting the degrees of protection afforded against C. jejuni invaded (Zheng et al., 2007).
• Efficacy: The chitosan-DNA vaccines induced strongest level of protection in BALB/c mice against challenge with C. jejuni HS:19 strain and the protective efficacies was 93.70 (Zheng et al., 2007).