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Vaccine Detail
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MUC1 24-mer Peptide-Loaded PLGA Microsphere Vaccine |
| Vaccine Information |
- Vaccine Name: MUC1 24-mer Peptide-Loaded PLGA Microsphere Vaccine
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Peptide vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: 24-mer MUC1 (TAPPAHGVTSAPDTRPAPGSTAPP) (Newman et al., 1998)
- Immunization Route: subcutaneous injection
- Description: A synthetic 24-mer MUC1 mucin peptide (TAPPAHGVTSAPDTRPAPGSTAPP) vaccine encapsulated in biodegradable PLGA microspheres (500–900 nm diameter) with MPLA as adjuvant, administered subcutaneously in C57BL/6J mice. Microspheres were prepared via water/oil/water solvent evaporation with 12% encapsulation efficiency and 0.02% w/w peptide loading. The vaccine was designed to prime antigen-specific Th1 responses without traditional adjuvants or carrier proteins, exploiting the capacity of PLGA microencapsulation to protect weakly immunogenic peptide antigens and facilitate uptake by phagocytic antigen-presenting cells. MPLA was co-encapsulated to further enhance Th1 polarization. Vaccination induced antigen-specific Th1-polarized immune responses with strong T cell proliferation against both the immunizing MUC1 24-mer and relevant MUC1 30-mer, with stimulation indices of 22 and 13 respectively without MPLA, increasing to 29 and 28 with MPLA, and no cross-reactivity to irrelevant antigens. The cytokine profile was dominated by high IFN-γ production with a ~20-fold increase with MPLA incorporation, and undetectable IL-4 and IL-10, confirming Th1 polarization. Without MPLA, no IgM or IgG responses were detected; with MPLA, a minimal IgG response (titer: 1/160) was observed, predominantly IgG2b with lower levels of IgG2a and IgG3, and absent IgG1. MPLA-only microspheres failed to elicit any immune response, confirming that immunogenicity required the peptide antigen (Newman et al., 1998).
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| Host Response |
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| References |
Newman et al., 1998: Newman KD, Sosnowski DL, Kwon GS, Samuel J. Delivery of MUC1 mucin peptide by Poly(d,l-lactic-co-glycolic acid) microspheres induces type 1 T helper immune responses. Journal of pharmaceutical sciences. 1998; 87(11); 1421-1427. [PubMed: 9811500].
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