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Vaccine Detail
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hu(TA)MUC1 Glycopeptide (STn-T3/Tn-T16)–TTox Conjugate Vaccine |
| Vaccine Information |
- Vaccine Name: hu(TA)MUC1 Glycopeptide (STn-T3/Tn-T16)–TTox Conjugate Vaccine
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Conjugate vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Stergiou et al., 2017)
- Immunization Route: Intraperitoneal injection (i.p.)
- Description: A synthetic glycopeptide vaccine containing a 22-amino acid human tumor-associated MUC1 (hu(TA)MUC1) tandem repeat peptide with sialyl-Tn (STn) antigen at threonine-3 and Tn antigen at threonine-16, conjugated to tetanus toxoid (TTox) via squaric acid chemistry. The vaccine targets aberrantly glycosylated MUC1 expressed on adenocarcinomas. Vaccination stimulates similar strong humoral immune responses in wild-type and huMUC1-transgenic mice with IgG1 and IgG2b antibodies (endpoint titers: 5×10⁵ and 5×10⁴ respectively); huMUC1-transgenic mice showed elevated IgM indicating reduced Th-mediated class switching due to partial tolerance. Antisera bound tumor-associated MUC1 on MCF-7 breast cancer cells (10.8–25.4% in wild-type; 4.58–18.2% in transgenic mice) with similar binding intensity, and significantly activated B cells, CD4⁺ T cells, and dendritic cells (p≤0.01–0.001). TTox provides T-helper cell epitopes sufficient to break immune tolerance in huMUC1-transgenic mice expressing MUC1 as a self-antigen, without inducing autoimmunity. MUC1 antigen, a membrane-bound glycoprotein expressed by most glandular and ductal epithelial cells, is overexpressed in an aberrant or deglycosylated form in breast, pancreatic, prostate, stomach, colon, and ovarian cancers (Stergiou et al., 2017).
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| Host Response |
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| References |
Stergiou et al., 2017: Stergiou N, Glaffig M, Jonuleit H, Schmitt E, Kunz H. Immunization with a Synthetic Human MUC1 Glycopeptide Vaccine against Tumor-Associated MUC1 Breaks Tolerance in Human MUC1 Transgenic Mice. ChemMedChem. 2017; 12(17); 1424-1428. [PubMed: 28675699].
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