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Vaccine Detail
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Four-Component MUC1-diTn Conjugate Vaccine with Three T-Helper Epitopes |
| Vaccine Information |
- Vaccine Name: Four-Component MUC1-diTn Conjugate Vaccine with Three T-Helper Epitopes
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Conjugate vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Palitzsch et al., 2014)
- Immunization Route: Intraperitoneal injection (i.p.)
- Description: A fully synthetic four-component antitumor vaccine consisting of a tumor-associated MUC1 glycopeptide (22-mer) bearing two TN antigens (GalNAc-?-O) at threonine-11 and serine-17, covalently conjugated to three different bacterial T-helper cell epitopes via squaric acid linkage, with potential antineoplastic activity. The vaccine includes a 14-mer from Yersinia pestis, a 15-mer from Yersinia pestis, and a 20-mer from Mycobacterium tuberculosis as T-helper epitopes, separated by flexible triethylene glycol spacers. Vaccination elicits adaptive T-cell-mediated immune responses with predominantly IgG1 antibodies that recognize tumor-associated MUC1 on human breast cancer cells. In preclinical studies, the four-component vaccine induced antibody titers approximately 8-fold higher than vaccines containing a single T-helper epitope (~30,000 vs ~4,000 endpoint titers), with 71% binding to MUC1-expressing T47D breast cancer cells and formation of immunological memory. MUC1 antigen, a membrane-bound glycoprotein expressed by most glandular and ductal epithelial cells, is overexpressed in an aberrant or deglycosylated form in various cancers such as those of the breast, prostate, pancreas, and ovary (Palitzsch et al., 2014).
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| Host Response |
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| References |
Palitzsch et al., 2014: Palitzsch B, Hartmann S, Stergiou N, Glaffig M, Schmitt E, Kunz H. A fully synthetic four-component antitumor vaccine consisting of a mucin glycopeptide antigen combined with three different T-helper-cell epitopes. Angewandte Chemie (International ed. in English). 2014; 53(51); 14245-14249. [PubMed: 25318465].
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