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Vaccine Detail
B. pertussis FhaB and 69 kDa OMP protein vaccine |
Vaccine Information |
- Vaccine Name: B. pertussis FhaB and 69 kDa OMP protein vaccine
- Target Pathogen: Bordetella pertussis
- Target Disease: Whooping Cough
- Vaccine Ontology ID: VO_0011498
- Type: Subunit vaccine
- Status: Research
- Antigen: Combination of the B. pertussis 69-kDa outer membrane protein and filamentous hemagglutinin (fhaB)
- fhaB
gene engineering:
- Type: Recombinant protein preparation
- Description: A crystal clear supernatant of 5-day-old static cultures containing proteolysis inhibitor was purified. To remove impurities, the FHA was precipitated by overnight dialysis against 25-30 volumes of buffer A at 4 C, and the precipitate was collected by centrifugation. The precipitated FHA was dissolved in the smallest possible volume of 40 mM B-alanine buffer at pH 3.5, clarified by centrifugation, and applied at room temperature at a flow of 30 ml/h to a Superose 12 column. The retained highly purified FHA was eluted (Novotny et al., 1991).
- Detailed Gene Information: Click Here.
- Adjuvant:
- Immunization Route: Intramuscular injection (i.m.)
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Host Response |
Mouse Response
- Vaccination Protocol: Groups of mice were injected intraperitoneally with 0.5 ml volumes of four concentrations (Novotny et al., 1991).
- Challenge Protocol: Mice were challenged intracerebrally 14 days after antigen injection. The challenge dose contained 300-400 LD50, and a 1:1250 dilution of the challenge does contained ~ 10cfu. All mice were challenged within 90 minutes after the challenge suspension was thawed; the challenge was injected using a 1 ml syringe in a Hamilton PB600-I dispensor that reproducibly delivers 20-ul voumes (Novotny et al., 1991).
- Efficacy: A combination of the 69-kDa outer membrane protein and filamentous hemagglutinin (fhaB), both isolated from lymphocytosis promoting factor (LPF; pertussis toxin) minus mutants of Bordetella pertussis, is protective in the mouse intracerebral challenge potency (Kendrick) test (Novotny et al., 1991).
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References |
Novotny et al., 1991: Novotny P, Chubb AP, Cownley K, Charles IG. Biologic and protective properties of the 69-kDa outer membrane protein of Bordetella pertussis: a novel formulation for an acellular pertussis vaccine. The Journal of infectious diseases. 1991; 164(1); 114-122. [PubMed: 2056199].
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