• Vaccine Name: V. cholerae CTB DNA Vaccine
• Target Pathogen: Vibrio cholerae
• Target Disease: Cholera
• Vaccine Ontology ID: VO_0011360
• Type: DNA vaccine
• Status: Research
• Antigen: cholera toxin B subunit (CTB) (Xu et al., 2009).
• ctxB gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000276
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: BALB/c mice
• Vaccination Protocol: Mice were immunized intramuscularly with 100 μg of the CTB DNA vaccine or vector control. The DNA immunizations for mice were given at Weeks 0 and 2 in DNA alone prime boost groups, or Week 0 only for those receiving DNA prime followed by KWC-B vaccine boost at Week 2 (Xu et al., 2009).
• Challenge Protocol: Naïve suckling BALB/C mice at 5- to 6-day-old were separated from their mothers for 4 h, then inoculated by oral gavage with 50 μl of the appropriate serial dilution of the inoculum. To prepare the inoculum, V. cholerae O1 El Tor biotype Ogawa strain V48-65 growing overnight on an LB plate, were re-suspended in PBS and adjusted to 5 LD50 in 25 μl, then incubated with 25 of properly diluted immune or normal rabbit serum samples for 1 h at 37 °C. The suckling mice were kept in a 25 °C environment and observed every 4 h for 48 h post-inoculation. The survival rates were documented at each time point (Xu et al., 2009).
• Efficacy: The immune sera elicited by CTB DNA vaccine were functional and protective against V. cholerae O1 infection in mice (Xu et al., 2009).