• Vaccine Name: B. bovis GST-12D3
• Target Pathogen: Babesia bovis
• Target Disease: Babesiosis, Texas Cattle Fever
• Vaccine Ontology ID: VO_0011375
• Type: Subunit vaccine
• Status: Research
• Antigen: B. bovis T2Bo 12D3
• 12D3 gene engineering:
  • Type: Recombinant protein preparation
  • Description: The 12D3 protective antigen was cloned and expressed as a glutathione-S-transferase (GST) fusion protein (Wright et al., 1992).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
• Immunization Route: Intramuscular injection (i.m.)

Cattle Response

• Vaccination Protocol: 50 micrograms of the GST-12D3 fusion protein was mixed with FCA and used to vaccinate cattle (Wright et al., 1992).
• Challenge Protocol: Challenged with virulent B.bovis (Wright et al., 1992).
• Efficacy: Immunised animals had parasitaemias one order of magnitude lower than controls when challenged with a heterologous strain of B. bovis. In a series of experiments, 100% of controls were treated, whereas only 16% of the GST-12D3 vaccinated animals required treatment (Wright et al., 1992).