• Vaccine Name: A. salmonicida live-attenuated vaccine encoding AroA
• Target Pathogen: Aeromonas salmonicida
• Target Disease: Furunculosis, Bald sea urchin disease
• Vaccine Ontology ID: VO_0011386
• Type: Live, attenuated vaccine
• Status: Research
• Antigen: A. salmonicida 644Rb aroA
• aroA gene engineering:
  • Type: Gene mutation
  • Description: The aroA gene of A. salmonicida was cloned in Escherichia coli, and the nucleotide sequence was determined. The aroA gene was inactivated by inserting a fragment expressing kanamycin resistance within the coding sequence. The aroA::Kar mutation was introduced into the chromosome of virulent A. salmonicida 644Rb and 640V2 by allele replacement by using a suicide plasmid delivery system. The mutation resulted in attenuation when bacteria were injected intramuscularly into Atlantic salmon (Vaughan et al., 1993).
  • Detailed Gene Information: Click Here.
• aroA gene engineering:
  • Type: Gene mutation
  • Description: This aroA mutant is from Aeromonas salmonicida (Vaughan et al., 1993).
  • Detailed Gene Information: Click Here.
• Immunization Route: Intraperitoneal injection (i.p.)

Brown Trout Response

• Vaccination Protocol: Brown trout were vaccinated with 10^7 CFU of A. salmonicida 644Rb aroa by intraperitoneal injection (Vaughan et al., 1993).
• Challenge Protocol: Groups of four or five fish were challenged by intramuscular injection of dilutions of cultures of a virulent strain of A. salmonicida (Vaughan et al., 1993).
• Efficacy: Vaccination of brown trout with 10^7 CFU of A. salmonicida 644Rb aroA by intraperitoneal injection resulted in a 253-fold increase in the 50% lethal dose (LD50) compared with unvaccinated controls challenged with a virulent clinical isolate 9 weeks later. A second vaccination after 6 weeks increased the LD50 by a further 16-fold (Vaughan et al., 1993).