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Vaccine Detail
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STn-MUC1-CRM197 Conjugate Vaccine with MPL/QS21 |
| Vaccine Information |
- Vaccine Name: STn-MUC1-CRM197 Conjugate Vaccine with MPL/QS21
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Conjugate vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Teng et al., 2025)
- Immunization Route: subcutaneous injection
- Description: A synthetic glycopeptide vaccine containing a 21-amino acid MUC1 tandem repeat sequence modified with sialyl-Tn (STn) glycan, conjugated to CRM197 carrier protein via squaric acid chemistry, and formulated with MPL and QS21 adjuvants. Vaccination with STn-MUC1-CRM197 conjugate plus MPL+QS21 adjuvants elicited the highest anti-MUC1 IgG titers among tested formulations, with elevated levels across all IgG subtypes and a potent Th1-biased immune response characterized by elevated IgG2a. The vaccine demonstrated 43.5% complement-dependent cytotoxicity (CDC) against MCF-7 breast cancer cells, the strongest binding to MCF-7 cells among formulations tested, and enhanced antibody-dependent cellular phagocytosis (ADCP) activity. Glycosylated vaccines showed superior CDC activity (41.5-47.5%) compared to non-glycosylated vaccine (37.1%), demonstrating tumor-specific binding to human breast cancer tissues and the ability to break immune tolerance. The STn antigen (sialyl-Thomsen-nouveau) is a tumor-associated carbohydrate antigen found in nearly 90% of breast cancers but almost undetectable in normal tissue. MUC1 antigen, a membrane-bound glycoprotein expressed by most glandular and ductal epithelial cells, is overexpressed in an aberrant or hypoglycosylated form in various epithelial cancers (Teng et al., 2025).
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| Host Response |
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| References |
Teng et al., 2025: Teng C, Ma W, Liu J, Hou J, Zhang Y, Meng X, Xue Y, Wang Z, Wang J, Chen D, Sui Q, Gao Q, Li X, Li T, Zong C. Chemoenzymatic liquid-phase synthesis and immunogenic assessment of tumor-associated complex MUC1 glycopeptide variants. International journal of biological macromolecules. 2025; 302; 140525. [PubMed: 39892541].
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