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Vaccine Detail
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MUC1-PD-L1-T helper epitope-IgG1 Fc Fusion Protein-Loaded DC Vaccine |
| Vaccine Information |
- Vaccine Name: MUC1-PD-L1-T helper epitope-IgG1 Fc Fusion Protein-Loaded DC Vaccine
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Dendritic cell vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Pan et al., 2022)
- Immunization Route: subcutaneous injection
- Description: A therapeutic dendritic cell vaccine loaded with a recombinant fusion protein (MUC1-Vax) containing human MUC1 extracellular domain, PD-L1 extracellular domain, T helper epitope sequence, and human IgG1 Fc fragment. The fusion protein is expressed in E. coli (ClearColi® BL21(DE3)), purified, and used to pulse syngeneic bone marrow-derived dendritic cells (BMDCs). The IgG1 Fc domain enhances antigen uptake by dendritic cells through Fcγ receptor-mediated endocytosis, improving cross-presentation to both MHC-I and MHC-II pathways. DCs are matured with LPS prior to administration. Vaccination elicits elevated anti-PD-L1 antibody production (significantly higher than PD-L1-only vaccine), increased CD4⁺ and CD8⁺ T cell activation with upregulated CD69 expression, and enhanced IFN-γ and IL-2 secretion, resulting in stronger CTL responses. In C57BL/6 mouse models bearing MUC1⁺ and PD-L1⁺ tumors (LLC-MUC1-PD-L1 and Panc02-MUC1-PD-L1), the vaccine significantly inhibited tumor growth, with complete tumor regression observed in some LLC-bearing mice, and prolonged survival compared to control groups. The dual-targeting MUC1-Vax vaccine demonstrated superior efficacy compared to PD-L1-only DC vaccine, providing a novel immunotherapy strategy for treating solid tumors with high MUC1 and PD-L1 expression (Pan et al., 2022).
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| Host Response |
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| References |
Pan et al., 2022: Pan J, Zeng W, Jia J, Shi Y, Wang D, Dong J, Fang Z, He J, Yang X, Zhang R, He M, Huang M, Fu B, Zhong B, Liu H. A Novel Therapeutic Tumor Vaccine Targeting MUC1 in Combination with PD-L1 Elicits Specific Anti-Tumor Immunity in Mice. Vaccines. 2022; 10(7); . [PubMed: 35891256].
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