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Vaccine Detail
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CpG-Loaded MUC1-Tn Glycopeptide/P2 Tetanus Epitope Conjugate Cationic Nanohydrogel Vaccine |
| Vaccine Information |
- Vaccine Name: CpG-Loaded MUC1-Tn Glycopeptide/P2 Tetanus Epitope Conjugate Cationic Nanohydrogel Vaccine
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Conjugate vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Hartmann et al., 2015)
- Immunization Route: Intraperitoneal injection (i.p.)
- Description: A self-adjuvanting conjugate vaccine containing tumor-associated MUC1-Tn glycopeptide (B-cell epitope) and P2 tetanus toxin-derived T-helper-cell epitope covalently conjugated onto CpG-loaded cationic nanohydrogel particles, with potential antitumor activity. Vaccination may stimulate humoral immune responses and T-cell proliferation against MUC1-expressing tumor cells without external adjuvants. In vivo studies in Balb/c mice (n=3/group) demonstrated rapid IgG antibody production after first boost with CpG-loaded vaccine versus five boosts required without CpG, both without CFA; no MUC1-specific antibodies detected without CpG after first boost; only CpG-loaded vaccine-induced antibodies showed binding to MUC1-expressing T47D human breast cancer cells with superior affinity; both vaccines induced T-helper-cell-mediated responses. CpG oligonucleotide complexed within the cationic nanohydrogel core activates intracellular TLR9, upregulating costimulatory markers CD40, CD80, and CD86 on dendritic cells to LPS-equivalent levels. MUC1 antigen, bearing truncated tumor-associated Tn carbohydrate antigen, is overexpressed in an aberrant form in epithelial cancers including breast cancer (Hartmann et al., 2015).
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| Host Response |
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| References |
Hartmann et al., 2015: Hartmann S, Nuhn L, Palitzsch B, Glaffig M, Stergiou N, Gerlitzki B, Schmitt E, Kunz H, Zentel R. CpG-loaded multifunctional cationic nanohydrogel particles as self-adjuvanting glycopeptide antitumor vaccines. Advanced healthcare materials. 2015; 4(4); 522-527. [PubMed: 25327631].
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