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Vaccine Detail
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Plant-Expressed Hydroxyproline (Hyp) Tri-Arabinan (Ara₃)-Glycosylated Heat-Labile Enterotoxin B Subunit (LTB)–MUC1 Vaccine with CpG 1826 |
| Vaccine Information |
- Vaccine Name: Plant-Expressed Hydroxyproline (Hyp) Tri-Arabinan (Ara₃)-Glycosylated Heat-Labile Enterotoxin B Subunit (LTB)–MUC1 Vaccine with CpG 1826
- Target Pathogen: Cancer
- Target Disease: Cancer
- Type: Recombinant fusion protein vaccine
- Status: Research
- Host Species for Licensed Use: Mouse
- Antigen: MUC1 (Pinkhasov et al., 2011)
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Adjuvant:
CpG ODN 1826 (Pinkhasov et al., 2011)
- Immunization Route: Intraperitoneal injection (i.p.)
- Description: A peptide vaccine containing the MUC1 tumor-associated tandem repeat peptide fused to heat-labile enterotoxin B subunit (LTB), produced in Nicotiana benthamiana plants using the magnICON™ viral expression system. The MUC1 peptide undergoes plant-specific O-linked hydroxyproline arabinosylation with tri-arabinan, essential for immune recognition and antigenicity. Vaccination of MUC1 transgenic mice with LTB-MUC1-6His plus CpG ODN adjuvant successfully broke self-antigen tolerance, inducing anti-MUC1 IgG, IgG1, and IgG2a antibody responses with significantly higher titers compared to controls (P < 0.05). CpG ODN shifted the immune response from Th2 bias toward Th1. This is the first plant-derived breast cancer antigen to demonstrate breach of tolerance in a transgenic mouse model. LTB serves as a carrier protein with mucosal-targeting and immunomodulatory properties. MUC1 antigen, a membrane-bound glycoprotein expressed by most glandular and ductal epithelial cells, is overexpressed and aberrantly glycosylated in various cancers including breast, prostate, and ovarian cancers (Pinkhasov et al., 2011).
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| Host Response |
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| References |
Pinkhasov et al., 2011: Pinkhasov J, Alvarez ML, Rigano MM, Piensook K, Larios D, Pabst M, Grass J, Mukherjee P, Gendler SJ, Walmsley AM, Mason HS. Recombinant plant-expressed tumour-associated MUC1 peptide is immunogenic and capable of breaking tolerance in MUC1.Tg mice. Plant biotechnology journal. 2011; 9(9); 991-991001. [PubMed: 21740504].
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