• Vaccine Name: AHB-SeNPs
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Mouse
• Antigen: AHB fusion protein composed of Ag85A, HspX, and bovine neutrophil ?-defensin-5 (B5).
• FbpA (Ag85A) gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Ge et al., 2024).
  • Detailed Gene Information: Click Here.
• hspX gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Ge et al., 2024).
  • Detailed Gene Information: Click Here.
• DEFB5 gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Ge et al., 2024).
  • Detailed Gene Information: Click Here.
• Preservative: Selenium nanoparticles were synthesized by reducing sodium selenite with ascorbic acid, using polyvinyl pyrrolidone (PVP K30) as a stabilizer.The AHB fusion protein was expressed in E. coli, purified, and endotoxin was removed. The vaccine was prepared by mixing AHB protein with SeNPs in PBS prior to intranasal administration (Ge et al., 2024).
• Immunization Route: Nasal spray

Mouse Response

• Host Strain: C57BL/6
• Vaccination Protocol: Mice received three intranasal immunizations at three-week intervals. Each dose consisted of 20 µg AHB fusion protein formulated with 20 µg selenium nanoparticles (SeNPs) in PBS (Ge et al., 2024).
• Challenge Protocol: Mice were intranasally challenged with ~100 CFU of Mycobacterium bovis (Beijing strain C68004) three weeks after the final immunization. Bacterial burden and pathology were assessed 4 weeks post-challenge (Ge et al., 2024).
• Efficacy: Vaccinated mice showed significantly reduced bacterial loads in lungs and spleen. Lung inflammation and pathological lesions were markedly decreased compared to controls (Ge et al., 2024).
• Description: In a C57BL/6 mouse model, the vaccine induced robust Th1/Th17 cellular immunity, mucosal antibody responses, and lung-resident memory T cells, resulting in significant protection against Mycobacterium bovis infection (Ge et al., 2024).
• Information about this animal model: Mouse Model for TB research