• Vaccine Name: BG–Lentinan/Mn(J)
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Type: Subunit vaccine
• Status: Research
• Host Species for Licensed Use: Rabbit
• Antigen: Fusion protein BG (BfrB–GrpE) derived from Mycobacterium tuberculosis (Zhou et al., 2025).
• bfrB gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Zhou et al., 2025).
  • Detailed Gene Information: Click Here.
• bfrB gene engineering:
  • Type: Fusion Protein
  • Description: This is use in fusion protein preparation (Zhou et al., 2025).
  • Detailed Gene Information: Click Here.
• Preparation: The BG fusion protein was recombinantly expressed in E. coli, purified by ammonium sulfate precipitation and hydrophobic interaction chromatography, and formulated with lentinan alone and lentinan plus Mn(J) in PBS for subcutaneous immunization (Zhou et al., 2025).
• Immunization Route: subcutaneous injection
• Description: BG–Lentinan/Mn(J) is a fusion protein subunit vaccine combining the BfrB–GrpE fusion protein with lentinan and a manganese-based adjuvant, which enhances antibody responses and accelerates lesion healing, but does not surpass BG–Lentinan alone in bacterial clearance (Zhou et al., 2025).

Rabbit Response

• Host gender: female
• Vaccination Protocol: Rabbits received subcutaneous immunization with BG–Lentinan/Mn(J) at weeks 0, 2, and 4, with each 1000 µL dose containing 100 µg of BG fusion protein, 500 µg of lentinan, and 500 µg of Mn(J)(Zhou et al., 2025).
• Challenge Protocol: Rabbits were challenged six weeks after the final immunization by intradermal injection of 5 × 10⁶ CFU of Mycobacterium bovis BCG into the dorsal skin (Zhou et al., 2025).
• Efficacy: The BG–Lentinan/Mn(J) vaccine significantly reduced bacterial burden and accelerated lesion healing, showing protective efficacy comparable to BCG vaccination (Zhou et al., 2025).
• Description: BG–Lentinan/Mn(J) is a tuberculosis protein subunit vaccine that combines the BfrB–GrpE fusion protein with lentinan and a manganese-based adjuvant to enhance immunogenicity and protective efficacy in rabbits (Zhou et al., 2025).